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Guangzhou Dongsheng Biotech Co., Ltd

City:guangzhou

Province/State:guangdong

Country/Region:china

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DNase I, RNase-Free Molecular Tool Enzyme

Name: DNase I RNaseFree Molecular Tool Enzyme
Brand: GDSBio
Price: 76 USD

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Brand Name :GDSBio

Model Number :E1018

Certification :/

Place of Origin :China

MOQ :1000 U

Price :USD 76-84.5/1000 U

Payment Terms :L/C, D/A, D/P, T/T, Western Union, MoneyGram

Supply Ability :100 Bag/Bags Per Day

Delivery Time :8 work days

Packaging Details :small package or bulk distribute or OEM

Product Name :DNase I, RNase-Free, HC

Cat. No./Spec. :E1018-A/1,000 U

Appearance :colourless

Application :cleave both single-stranded and double-stranded DNA

Classification :General Reagents

Grade :molecular biology

Logo Printing :With Logo Printing

Transport Package :Packing

Production Capacity :100 Bag/Bags Per Day

Storage Conditions :Store at -20°C

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DNase I, RNase-Free, HC

Cat. No./Spec.: E1018-A/1,000 U
Concentration: 50 U/µL

Product Description
DNase I (without RNase) is an endonuclease that can cleave both single-stranded and double-stranded DNA. It hydrolyzes
phosphodiester bonds, producing single deoxyribonucleotides and oligodeoxyribonucleotides with 5'-phosphate groups and 3'-OH groups.
The activity of this enzyme is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions. In the presence of Mg2+, DNase I cuts each strand of dsDNA in a statistically random and independent manner. When Mn2+ is present, the enzyme almost cuts both DNA strands at the same site, resulting in DNA fragments with blunt ends or overhangs of one or a few nucleotides.

Storage Condition & Shelf Life
Store at -20°C.

Source
Recombinant E. coli strain containing the cloned gene encoding bovine DNase I.

Unit Definition
A unit is defined as the amount of enzyme required to completely degrade 1 µg of plasmid DNA within 1 minute at 37°C.
Enzyme activity is determined in the following mixture: 10 mM Tris-HCl (pH 7.5 at 25°C), 2.5 mM MgCl2, 0.1 mM CaCl2, and 1 µg of pUC19 DNA.
One unit of DNase I is equivalent to 0.3 Kunitz units.

Features
- Recombinant enzyme
- Purified from a non-animal host, with low levels of endogenous RNase

Scope of Application
- For the preparation of DNA-free RNA.
- To remove template DNA after in vitro transcription.
- To prepare DNA-free RNA before RT-PCR and RT-qPCR.
- In conjunction with DNA Polymerase I for DNA labeling through nick translation.
- For the study of DNA-protein interactions using DNase I (RNase-free) footprinting.
- To generate a library of randomly sheared DNA insert fragments. Reaction buffer containing Mn2+ is used.

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