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Reverse Transcriptase PCR Reagents

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Guangzhou Dongsheng Biotech Co., Ltd

City:guangzhou

Province/State:guangdong

Country/Region:china

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Molecular Biology Grade S1 Nuclease

Name: Molecular Biology Grade S1 Nuclease
Brand: GDSBio
Price: 58 USD

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Brand Name :GDSBio

Model Number :E1017

Certification :/

Place of Origin :China

MOQ :10000 U

Price :USD 58-64.5/10000 U

Payment Terms :L/C, D/A, D/P, T/T, Western Union, MoneyGram

Supply Ability :100 Bag/Bags Per Day

Delivery Time :8 work days

Packaging Details :small package or bulk distribute or OEM

Product Name :S1 Nuclease

Cat. No./Spec. :E1017-A/10,000 U

Appearance :colourless

Application :degrade single-stranded nucleic acids

Classification :General Reagents

Grade :molecular biology

Logo Printing :With Logo Printing

Transport Package :Packing

Production Capacity :100 Bag/Bags Per Day

Storage Conditions :Store at -20°C

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S1 Nuclease

Cat. No./Spec.: E1017-A/10,000 U
Concentration: 100 U/µL

Product Description
S1 nuclease can degrade single-stranded nucleic acids, releasing 5'-phosphorylated mononucleotides or oligonucleotides. Its activity on DNA is five times that on RNA. S1 nuclease can also cleave double-stranded DNA (dsDNA) at single-stranded regions caused by nicks, gaps, mismatches, or loops. S1 nuclease exhibits 3'-phosphomonoesterase activity.
This enzyme is a glycoprotein with a carbohydrate content of 18%.

Storage Condition & Shelf Life
Store at -20°C.

Source
Aspergillus oryzae strain.

Unit Definition
A unit is defined as the amount of enzyme required to produce 1 µg of acid-soluble deoxyribonucleotides in 1 minute at 37°C. Enzyme activity is determined in the following mixture: 30 mM sodium acetate (pH 4.5), 50 mM NaCl, 0.1 mM ZnCl2, 5% (v/v) glycerol, and 800 µg/mL heat-denatured calf thymus DNA.

Scope of Application
- Removal of single-stranded overhangs from DNA fragments
- Localization of S1 transcripts
- Cleavage of hairpin loops
- In conjunction with Exonuclease III, creating unidirectional deletions within DNA fragments

Inhibition and Inactivation
- Inhibitors: metal chelating agents, PPi (inorganic pyrophosphate), Pi (inorganic phosphate), 5'-ribonucleotides, and
deoxyribonucleotides.
- Inactivate by heating at 70°C for 10 minutes in the presence of EDTA.

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