RNase H
Cat. No./Spec.: E1016-A/100 U, E1016-B/500 U
Concentration: 5 U/µL
Product Description
Ribonuclease H (RNase H) can specifically degrade the RNA strand within RNA-DNA hybrids. This enzyme does not hydrolyze the phosphodiester bonds in single-stranded or double-stranded DNA and RNA.
Storage Condition & Shelf Life
Store at -20°C.
Source
E. coli strain MRE-600.
Unit Definition
A unit is defined as the amount of enzyme required to catalyze the formation of 1 nmol of acid-soluble products within 20 minutes at 37°C.
Enzyme activity is determined in the following mixture: 20 mM Tris-HCl (pH 7.8), 40 mM KCl, 8 mM MgCl2,1 mM DTT, 24 µM [3H]-poly(A)·poly(dT), 0.03 mg/mL BSA, and 4% (v/v) glycerol.
Scope of Application
- Removal of mRNA before the synthesis of the second-strand cDNA
- RT-PCR and qRT-PCR: Removal of RNA after the first-strand cDNA synthesis
- Removal of poly(A) sequence from mRNA after hybridization with Oligo(dT)
- Site-specific cleavage of RNA
- Study of products from in vitro polyadenylation reactions
