Guangzhou Dongsheng Biotech Co., Ltd
Professional Supplier of PCR & NGS Reagents
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Lyticase
Components
| Component | N9031 | N9032 |
| Lyticase | 150ul | 300ul |
| Buffer | 10ml | 20ml |
Recommend use concentration: 0.15U/μl
Store at -20°C
For research use only
Description
Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.
Applications
Biochem/physiol Actions
Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.
Definition of Activity Unit
One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25°C, using a suspension of yeast as substrate in a 3 ml reaction mixture.
Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.