Guangzhou Dongsheng Biotech Co., Ltd
Professional Supplier of PCR & NGS Reagents
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High quality Swab/Saliva Viral DNA/RNA Extraction Kit (Spin Column) V4001
Catalog No.: V4001 Size: 100 preps
Description
The Swab/Saliva Viral DNA/RNA Extraction Kit (Spin Column) is suitable for rapid extraction of high purity viral nucleic acid from plasma, serum, nasopharyngeal swab, sputum, bronchus/alveolar lavage fluid, ascites, culture cell supernatant and urine. This kit is based on the silica gel column purification method. The sample is homogenized in the lysis buffer and the nucleic acid is released into the buffer. The lysis buffer contains a high concentration of guanidine. In this condition, the membrane absorbs nucleic acid by hydrogen bond and electrostatic physical and chemical action, while proteins and other impurities are not absorbed. The lysate is transferred to the adsorption column for filtration, and the nucleic acid filter membrane is washed to remove residual proteins and other impurities, and finally eluted by the low-salt buffer solution. The obtained nucleic acids can be directly used in downstream related experiments such as reverse transcription, PCR, RT-PCR, fluorescence quantitative PCR, second-generation sequencing and Northern hybridization.
Main components
The kit consists of the following components:
| The name of the reagent | Amount | Component description |
| Lysis Buffer | 50 ml | Provide environment for lysing and binding to the column |
| Wash Buffer | 24 ml | Remove residual proteins and other impurities |
| Elute Buffer | 6 ml | Nuclease-free solution |
| Spin Column | 50 pcs × 2 | Adsorb viral nucleic acid and collect the filtrate |
Storage conditions
Store at room temperature (15-25°C) and transport at room temperature.
Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.