Guangzhou Dongsheng Biotech Co., Ltd
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Heat Labile UDG
For research use only
Heat Labile UDG Highly effective anti-contamination enzyme R5001
Components
| Component | R5001 (500 U) | R5002 (100 U) |
| Heat Labile UDG (1 U/μl) | 500 μl | 100 μl |
Storage
This reagent should be kept at -20°C.
Description
Heat Labile UDG is purified from the expression product of an recombinant E. coli strain containing the UDG gene cloned from cold-adaptive marine bacteria (psychrophilic Marine bacterium). UDG (Uracil-DNA Glycosylase) catalyzes the hydrolysis of uracil bases of the single or double stranded DNA containing dU and the N-glycoside bonds of the glycosphosphoric acid skeleton to release free uracil. The resulting base-free site is easily fractured by hydrolysis. This product is sensitive to high temperature, and above 50°C can make the enzyme irreversible inactivation, suitable for PCR, qPCR, RT-PCR, RT-qPCR system.
Storage Buffer
20 mM Tris-HCl, pH 7.5 at 25°C
0.1 mM EDTA
100 mM KCl
1 mM DTT
Glycerol 50% (v/v)
CA630 0.5% (v/v)
0.5% Tween - 20 (v/v)
Source
Psychrophilic Marine Bacteium derived Heat Labile UDG expressed and purified in E. coli.
Unit Definition
In 70 mM Tris-HCl, pH 7.5, 10 mM NaCl, 1 mM EDTA, 100 g/ml BSA reaction solution, the amount of enzyme required to release 1 nmol uracil from DNA containing dU within 1 h at 37°C was defined as 1 unit of activity (U).
Thermal inactivation
95°C, 2 min
Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.