HS Mix PCR Master Mix #P2082 5ml

HS^TM Mix

#P2082, 5 ml

Contents:

Store at -20°C

For research use only.

In total 2 vials.

Description

2× HS^TM Mix is a premixed, ready-to-use solution containing HS^TM Taq DNA Polymerase, dNTPs, Mg²⁺ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. HS^TM Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes to higher specificity by optimizing the system, reducing primer-dimer rate.

HS^TM Taq DNA Polymerase is a thermostable recombinant DNA Polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. HS^TM Taq DNA Polymerase can amplify DNA target up to 5 kb. The elongation velocity is 2kb/min. It has 5' to 3' polymerase activity, but lacks of 3' to 5' exonuclease activity that results in 3'-dA overhangs PCR products. All components of the HS^TM Mix are at optimal concentration for efficient amplification. It contributes to highly specific incorporation of primers and template.

Composition of the 2× HS^TM Mix

0.3U/µl HS^TM Taq DNA Polymerase, 2× HS^TM PCR Buffer, 0.4mM dNTPs, 3.2mM MgCl_2, 0.02% bromophenol blue.

HS^TM Mix buffer is a proprietary formulation optimized for robust performance in PCR.

Applications

• High throughput PCR

• High specificity PCR

• Routine PCR with high reproducibility

• Generation of PCR products for TA cloning

Features

• Convenient: only primers and template DNA are added

when prepare final PCR

• High yields of PCR products with minimal optimization

• High efficiency: saving your time by simplifying the process

• Reproducible: lower contamination and pipetting error risk

• High sensitivity: compared to conventional Taq DNA Polymerase.

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.