Guangzhou Dongsheng Biotech Co., Ltd
Professional Supplier of PCR & NGS Reagents
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Pfu DNA Polymerase
Description
Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerase. Its molecular weight is 90 kDa. It can amplify DNA target up to 2 kb(simple template). The elongation velocity is 1kb/min(70~75°C). Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.
Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
Storage Buffer
20mM Tris-HCl (pH 8.0), 100mM KCl, 3mM MgCl2, 1mM DTT, 0.1% NP-40, 0.1% Tween20, 0.2mg/ml BSA, 50% (V/V) glycerol.
10× Pfu Buffer with Mg2+
200mM Tris-HCl (pH 8.8), 100mM KCl, 100mM (NH4)2SO4, 20mM MgSO4, 1% Triton X-100, 1mg/ml BSA.
Applications
• High-fidelity PCR and primer-extension reactions
• High fidelity PCR for cloning into blunt-ended vectors
• Site-directed mutagenesis
Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.